PCR Cycle Analysis

Fluorescence (510–570 nm) — 40-cycle PCR run

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LabSimple sensor CSVs (1 or more)

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QuantStudio XLSX

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Full Time Series

IN − OUT per cycle

For each cycle: mean signal during the in-chamber phase minus mean signal during the out-of-chamber phase. This differential removes baseline drift and directly tracks DNA amplification across cycles.

ΔRn axis max:

Cycle Overlay — all 40 cycles

Each line is one PCR cycle, aligned to the start of the in-chamber phase. The dashed line marks the transition to the out-of-chamber dip (shaded right). Colour transitions from faint (cycle 1) to solid (cycle 40). Rising signal level across later cycles reveals DNA amplification.